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Image Search Results
Journal: American Journal of Cancer Research
Article Title: Enhancing progestin therapy via HDAC inhibitors in endometrial cancer
doi:
Figure Lengend Snippet: Multiple HDAC inhibitors inhibit cell proliferation and restore functional PR expression in endometrial cancer cells. A. Cell proliferation assay: cell proliferation in Ishikawa, ECC1, and KLE endometrial cancer cell lines was evaluated after different HDACi treatments. B. Western blot analysis of PRB, PRA, FOXO1, p27, p21, Cyclin D1, Myc, histone H3 acetylation (H3Ace) and ERα in Ishikawa, ECC1 and KLE cells. β-actin serving as loading control. C. QPCR: ESR1, PGR, FOXO1, p21, AREG and PAEP mRNA expression normalized to 18S displayed as mean ± SD.
Article Snippet: Antibodies and reagents
Techniques: Functional Assay, Expressing, Proliferation Assay, Western Blot, Control
Journal: American Journal of Cancer Research
Article Title: Enhancing progestin therapy via HDAC inhibitors in endometrial cancer
doi:
Figure Lengend Snippet: HDACi treatment reverses PR and PAEP transcriptional repression. A. PR expression and binding of RNA polymerase II (RNA PII), H3K4Me3 and H3K27Me3 to the PR promoter region in the benign tissue (BE458) sample and two endometrial tumor samples (EA54 & EA68). *P < 0.05 vs. control. B, C. ChIP followed by q-PCR determined binding of H3K4Me3, H3K9Me3, RNA PII and SUZ12 to the different promoter regions in mice tumor samples in control (con C1) and romidepsin treated samples (Romi A1 & Romi B1). *P < 0.05 vs. control. B. In the PR promoter region. C. In the PAEP promoter region. D. ChIP followed by q-PCR determined the binding of RNA PII to the promoter regions of PR, PAEP, and Myc in Ishikawa mice tumor samples. *P < 0.05 vs. control. E. ChIP followed by q-PCR determined the binding of RNA PII to the Myc promoter region in control (con C1) and romidepsin treated mice tumors (Romi A1 & Romi B1).
Article Snippet: Antibodies and reagents
Techniques: Expressing, Binding Assay, Control
Journal: American Journal of Cancer Research
Article Title: Enhancing progestin therapy via HDAC inhibitors in endometrial cancer
doi:
Figure Lengend Snippet: Correlation of MPA + HDACi drug response genes
Article Snippet: Antibodies and reagents
Techniques: